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. 2020 Jul 21;10(7):1085. doi: 10.3390/biom10071085

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Time evolution of lipid mixing assay in GUVS bearing Mfn1-induced adhesion. Lipid mixing was monitored when fluorescence intensity was observed on the non-labelled vesicle after adhesion of two GUVs, one of them labelled with Rh-PE (0.5%mol). In 90% cases (N = 10) no fluorescence redistribution between vesicles was observed (top row). Only in one case, a fluorescent signal appeared in the non-labelled vesicle, suggesting lipid mixing (bottom row). Note that the equilibrium was only reached after 20–30 min. Scale bars are 10 μm.