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. 2020 Jun 28;12(7):1712. doi: 10.3390/cancers12071712

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Immunofluorescence of proteins stimulated by retinoic acid 6 (STRA6) and lecithin retinol acyltransferase (LRAT). (A–D) STRA6 labelling (green) is strong in the cytoplasm of urothelial cells of all groups. Scale bar = 50 μm. LRAT labelling (green) is present in the cytoplasm of the normal urothelial in (E) NT and (F) VitA groups. In (G) BBN and (H) BBN + VitA groups, LRAT labelling is strongest in the nuclei of urothelial cells (arrows). White line depicts the location of basal lamina, L, lumen. Scale bar = 50 μm.