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. 2020 Jun 29;12(7):1725. doi: 10.3390/cancers12071725

Figure 3.

Figure 3

Autophagy drives the potentiation of Ara-C cytotoxicity by DDA. HL-60 and KG1 cells were treated for 48 h with 5 µM DDA and 0.1 µM Ara-C, alone or in combination, in the absence or presence of (A) 40 µM z-VAD-fmk or (B) 5 nM bafilomycin A1 (Baf A1). Viability was measured by the Trypan Blue exclusion method. Bars are mean ±SEM of five independent experiments. (C) HL-60, and KG1 cells transfected with control shRNA (shCTRL) or shRNA against ATG5 (shATG5), ATG12 (shATG12) and against VPS34 (shVPS34) were treated for 48 h with 5 µM DDA and 0.1 µM Ara-C, alone or in combination. Viability was measured as described above. Bars are mean ± SEM of five independent experiments. * p < 0.05, ** p < 0.01, *** p < 0.001, n.s: non significant.