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. 2020 Jul 7;12(7):635. doi: 10.3390/pharmaceutics12070635

Figure 5.

Figure 5

Cellular uptake of niosome cargo in cultured primary rat astrocytes (RAC) after 4 h of incubation. (a) Uptake of cargo loaded in non-targeted (N) and alanine-glutathione targeted (N-A-GSH) niosomes. Values presented are means ± SEM. Statistical analysis: unpaired t test; ** p < 0.01; n = 6. (b) Effect of temperature and treatment with sodium-azide (1 mg/mL), filipin (5 µg/mL) or cytochalasin D (CD; 0.125 µg/mL) on the cellular uptake of cargo. Values presented are means ± SEM. Statistical analysis: one-way ANOVA followed by Dunnett’s posttest; ** p < 0.01, *** p < 0.001, ns: not significant (p > 0.05) compared to the first column of each niosome group; n = 6. (c) Live cell visualization of cargo taken up by astrocytes. Free cargo: cargo not loaded in niosomes. Bar: 25 µm.