Figure 4.

STL induced apoptosis in PCSC by disrupting the cell cycle progression. A, B. The percentage of cell death was determined by Annexin V-FITC/PI flow cytometry. The Annexin V-FITC/PI staining revealed that STL significantly induces cell death by apoptotic mechanism. C, D. Effects on cell cycle profile of PCSC after treatment with STL. Cells were treated with STL for 48 hrs and then fixed with 70% ice cold ethanol, and the DNA content was determined by flow cytometry analysis by PI staining, analyzing 50,000 events per sample. Results showed that majority of cells treated with STL accumulated in G₀ phase which is suggestive of dying cells. The data shown are representation of one of three independent experiments. Data are means ± SD (n = 3). *P<0.05; **P<0.01 compared to control.