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. 2020 Jul 1;10(7):2043–2065.

Figure 7.

Figure 7

STL down regulates the expression levels of stem cell markers of PCSC. For this, PCSC seeded onto 8-well chambers and treated with various doses of STL for 48 hrs and stained with ALDH1 and CD44, analyzed using confocal microscopy. Image analysis reveal that STL inhibits the expression of both ALDH1 and CD44 in PCSC. A, B. Confocal images of ALDH1 stain and quantification of ALDH1. C, D. Flow cytometric quantification of ALDH1. STL treated PCSC were stained with ALDH1-PE and subjected to flow analysis, and the results of which revealed that STL reduces the surface expression of ALDH1 in PCSC. E, F. Confocal images of CD44 and quantification of CD44. Magnification 60×. Scale bar 10 µm. Our data suggest that STL is more potent in inhibiting stemness of PCSC by targeting ALDH1 and CD44 stem cell markers. *P<0.05; **P<0.01 compared to control.