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. 2020 Jul 1;10(7):2083–2099.

Figure 3.

Figure 3

NTRK2 induces anoikis resistance in NPC cells. The NTRK2 inhibitor K252a (200 nM) was used to knock down NTRK2 activity in HM cells, and C666-1-shNTRK2 and CM-shNTRK2 cells with NTRK2 stably knocked down were used. (A) The expression of NTRK2, cleaved PARP and cleaved caspase3 was analyzed by western blot after suspension culture for 48 h. (B) NPC cells were grown under anchorage-free conditions for 24 h. Scale bar: 50 µm. (C) Fluorescence microscopy and (D) flow cytometric analysis were performed to investigate cell apoptosis after culture under suspension conditions for 24 h. Scale bar: 50 µm. (E) The viability of nonadherent cells was assessed by a trypan blue rejection experiment. (F) The colony numbers of NPC cells in soft agar (2 weeks) are shown. Scale bar: 100 μm. Columns: mean of three replicates; statistical significance was calculated using a t-test (*P<0.05, **P<0.01, and ***P<0.001).