Table 1.
Gene sequences of PCR primers
| Gene | Accession number | Sequence | Product Size (bp) | |
|---|---|---|---|---|
| 18SrRNA | X03205 | F | GTAACCCGTTGAACCCCATT | 151 |
| R | CCATCCAATCGGTAGTAGCG | |||
| β-actin | NM_001101 | F | ACTGGGACGACATGGAGAAA | 189 |
| R | ATAGCACAGCCTGGATAGCA | |||
| ASCT2 | NM_005628 | F | TCCTCTTCACCCGCAAAAACCC | 134 |
| R | CCACGCCATTATTCTCCTCCAC | |||
| ATB0,+ | NM_007231 | F | TGACCACTTCTGTGCTGGATGG | 155 |
| R | AGCAAGCTCTCCACCATAGCCA | |||
| BCAT2 | NM_001190 | F | CGCTGAATGGTGTTATCCTG | 109 |
| R | AACTGCTTCATGGTGATCGT | |||
| cMyc | NM_002467 | F | CCTGGTGCTCCATGAGGAGAC | 128 |
| R | CAGACTCTGACCTTTTGCCAGG | |||
| GLS1 | NM_001256310 | F | CAGAAGGCACAGACATGGTTGG | 117 |
| R | GGCAGAAACCACCATTAGCCAG | |||
| GLUT1 | NM_006516 | F | CTGCAACGGCTTAGACTTCGAC | 101 |
| R | TCTCTGGGTAACAGGGATCAAACA | |||
| GLUT3 | NM_006931 | F | TGCCTTTGGCACTCTCAACCAG | 98 |
| R | GCCATAGCTCTTCAGACCCAAG | |||
| HIF-1α | NM_001243084 | F | TATGAGCCAGAAGAACTTTTAGGC | 145 |
| R | CACCTCTTTTGGCAAGCATCCTG | |||
| HIF-1β | NM_001197325 | F | CTGTCATCCTGAAGACCAGCAG | 129 |
| R | CTGGTTCTCATCCAGAGCCATTC | |||
| LAT1 | NM_003486 | F | GCCACAGAAAGCCTGAGCTTGA | 136 |
| R | ATGGTGAAGCCGATGCCACACT | |||
| LAT3 | NM_003627 | F | ATGGACTGGCGGATCAAGG | 109 |
| R | TCTTGCAGTAGCGTGGTCTGATG | |||
| MCT1 | NM_003051 | F | TGGCACCAGGCAAACGAATA | 137 |
| R | AAATGCATCCAGCAATTCTAAGCAC | |||
| MCT2 | NM_001270623 | F | CCTGCGCCAGAGACCAGATAA | 109 |
| R | TTGGCACCAAGAGTCCCAGAG | |||
| PCK1 | NM_002591 | F | TGGATGAAGTTTGACGCACA | 124 |
| R | TGTTCTTCTGGATGGTCTTGAT | |||
| PCK2 | NM_004563 | F | TGACGCCATCATCTTTGGTG | 163 |
| R | CATGGCAAATGGGTCGTG | |||
| VEGFα | NM_001025366 | F | TTGCCTTGCTGCTCTACCTCCA | 126 |
| R | GATGGCAGTAGCTGCGCTGATA | |||
| xCT | NM_014331 | F | TCCTGCTTTGGCTCCATGAACG | 122 |
| R | AGAGGAGTGTGCTTGCGGACAT |
18SrRNA, 18S ribosomal RNA; ASCT2, system ASC transporter 2 (SLC1A5); ATB 0,+, amino acid transporter responsible for the activity of system B0,+ (SLC6A14); BCAT2, branched chain amino acid transaminase-2; cMYC, MYC proto-oncogene (bHLH transcription factor); GLS1, glutaminase 1; GLUT3, glucose transporter subtype 3 (SLC2A3); HIF-1α & β, hypoxia-inducible factor-1α & β; LAT1 & 3, L-type amino acid transporter 1 & 3 (SLC7A5 & SLC43A1); MCT1, 2, & 4, monocarboxylate transporter (SLC16A1, SLC16A7, & SLC16A4); PCK1, phosphoenolpyruvate carboxykinase 1; VEGFα, vascular endothelial growth factor α; xCT, system xc- transporter-related protein (SLC7A11); F, forward; R, reverse; bp, base pair.