Table 2.
Glucose and amino acid concentrations in the nontumor and tumor regions with and without diabetes mellitus type II
| Non-T2DM | T2DM | Two-way ANOVA analysis | |||||
|---|---|---|---|---|---|---|---|
|
|
|
|
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| Nontumor | Tumor | Nontumor | Tumor | Tu | DM | Tu × DM | |
| Glucose | 7.6±0.8 | 10.7±1.5 | 8.4±2.3 | 13.1±3.7 | n.s | n.s | n.s |
| Pyruvate precursor | |||||||
| Ser | 5.4±1.1 | 10.2±0.6 | 5.0±0.6 | 9.3±1.5 | ** | n.s. | n.s. |
| Gly | 3.9±0.7 | 22.3±1.9 | 5.1±1.3 | 23.8±4.3 | ‡ | n.s. | n.s. |
| Thr[s] | 2.6±0.6 | 6.6±0.4 | 2.7±0.5 | 6.7±1.0 | † | n.s. | n.s. |
| Ala | 15.9±2.9 | 41.1±4.0 | 13.4±2.0 | 33.8±4.1 | ** | n.s. | n.s. |
| Trp[aa] | 0.4±0.1 | 0.6±0.1 | 0.4±0.1 | 0.9±0.1 | † | n.s. | n.s. |
| Cys | 0.4±0.0 | 0.3±0.1 | 0.4±0.1 | 0.6±0.3 | n.s. | n.s. | n.s. |
| α-Ketoglutamate precursor | |||||||
| Gln | 5.1±1.1 | 17.3±2.2 | 5.4±1.1 | 13.9±3.4 | † | n.s. | n.s. |
| Glu | 2.8±0.8 | 42.6±5.6 | 4.5±1.6 | 32.1±9.4 | ‡ | n.s. | n.s. |
| Pro | 1.9±0.4 | 10.9±2.1 | 1.6±0.3 | 10.8±1.8 | † | n.s. | n.s. |
| Arg | 8.1±1.8 | 9.0±0.6 | 7.1±1.2 | 8.0±1.7 | n.s. | n.s. | n.s. |
| Succinyl-CoA precursor | |||||||
| Met | 1.7±0.3 | 2.7±0.2 | 1.8±0.3 | 2.7±0.5 | * | n.s. | n.s. |
| Ile[a] | 1.7±0.3 | 3.5±0.3 | 1.5±0.2 | 3.9±0.6 | † | n.s. | n.s. |
| Val | 4.0±0.7 | 11.7±1.1 | 4.4±0.9 | 12.5±1.3 | ‡ | n.s. | n.s. |
| Acetyl-CoA precursor | |||||||
| Leu | 6.1±1.2 | 7.8±0.5 | 5.8±0.8 | 8.3±1.1 | n.s. | n.s. | n.s. |
| Acetoacetyl-CoA precursor | |||||||
| Lys | 13.8±2.3 | 17.4±1.5 | 12.6±2.1 | 15.6±3.2 | n.s. | n.s. | n.s. |
| Phe[f] | 3.2±0.6 | 4.4±0.4 | 3.3±0.8 | 4.4±0.7 | * | n.s. | n.s. |
| Tyr[f] | 3.7±0.8 | 4.8±0.4 | 4.3±1.1 | 4.7±0.7 | n.s. | n.s. | n.s. |
Footnote: Data are shown as the mean ± SE of mg/g protein and nmol/mg protein in glucose and AAs, respectively. Amino acids marked with [a], [aa], [f], and [s] also belong to the categories of the precursors of acetyl-CoA, acetoacetyl-CoA, fumarate, and succinyl-CoA, respectively. *P<0.05, **P<0.01, †P<0.001, ‡P<0.0001, Abbreviations: n.s., no significant; T2DM, diabetes mellitus type II; Statistical differences were evaluated by two-way ANOVA analysis. Tu, ANOVA factor of tumor; DM, ANOVA factor of T2DM; Tu × DM, ANOVA factor of tumor and T2DM interaction.