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. 2020 Jun 28;12(7):1717. doi: 10.3390/cancers12071717

Figure 1.

Figure 1

Identification of focal adhesion proteins affecting cell survival, radiosensitivity and DNA repair. (A) Workflow of 3D high-throughput RNAi screening (3D HTP-RNAi-S); (B) Surviving fraction-related enhancement ratios of EGFP-53BP1-expressing UTSCC15 cell cultures in response to focal adhesion protein (FAP) knockdowns (n = 4), the data and p-values for which are provided in Table S2; (C) Surviving fraction-related enhancement ratios of FAP knockdown cell cultures exposed to 6-Gy X-rays (n = 4), the data and p-values for which are provided in Table S3; (D) Gain of residual 53BP1 foci number per cell (24 h after irradiation) in FAP knockdown cell cultures irradiated with 6-Gy X-rays (53BP1 foci in controls were subtracted from the total number of foci) (n = 4); (E) Scatter plot displaying the relation between 53BP1 residual foci/cell and surviving fraction upon FAP knockdown and 6-Gy irradiation. Main selected candidates with high foci/cell and low cell survival are indicated. SF, surviving fraction; CTRL, control.