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. 2020 Jun 28;12(7):1717. doi: 10.3390/cancers12071717

Figure 4.

Figure 4

Synemin/DNA-PKcs co-control DSB repair and radiation survival. (A) Knockdown efficiencies of single and double esi/siRNA transfections of SAS cells. β-actin served as loading control; (B,C) Residual 53BP1 and γH2AX foci per cell (24 h after irradiation) upon single and double knockdown of synemin and DNA-PKcs in 6-Gy X-ray irradiated SAS cells. Transfection with single or double non-specific siRNA were used as controls; (D) Plating efficiency of SAS cells upon single and double knockdown of synemin and DNA-PKcs; (E) 3D colony formation ability upon single and double silencing of synemin and DNA-PKcs. Data are represented as mean ± SD (n = 3; two-sided t-test; * p < 0.05, ** p < 0.01, *** p < 0.001; n.s., not significant (p ≥ 0.05)).