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. 2020 Jun 28;12(7):1717. doi: 10.3390/cancers12071717

Figure 5.

Figure 5

DNA-PKcs directly interacts with synemin and depends on ATM activity. (A) Immunoprecipitation (IP) of Synemin 1 h post 6-Gy X-rays. Western blot analysis showing expression of Synemin and DNA-PKcs in immunoprecipiates and whole cell lysates (WCL) from SAS cells; (B) Western blot on mCherry immunoprecipitates from 6-Gy irradiated mCherry-SAS and mCherry–Synemin-SAS cells at 1 h post irradiation. β-actin served as loading control; (C) Western blots on synemin immunoprecipitates from SAS and SKX cells 1 h after 6-Gy X-ray exposure; (D,E) 53BP1 and DNA-PKcs S2056 foci upon ATMi treatment (DMSO used as control) at different time points post 1-Gy X-rays in SAS mCherry and mCherry–Synemin transfectants. Data are represented as mean ± SD (n = 3; One-way ANOVA followed by post hoc test (Tukey multiple comparisons); * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; n.s., not significant (p ≥ 0.05)).