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ELISA analysis of the IL-8 knockdown efficiency of (A) PEI and (B) PEI-LPEG siRNA nanoparticles following direct administration onto fully polarised Calu-3 monolayers at N/P = 15. (C) Analysis of trans-epithelial electrical resistance (TEER) fluctuations in PEI and PEI-LPEG siRNA nanoparticle (N/P = 15) over 24 h (significance vs. PBS-treated samples, 1 and 2-way ANOVA, n = 3 ± SD, * p < 0.05, ** p < 0.01, *** p < 0.001).
