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. 2020 Aug 6;20:585. doi: 10.1186/s12879-020-05317-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Examination of the effect of UV irradiation on IAV infectivity by plaque assay. IAV suspensions were irradiated with a UV lamp for increasing periods of time between 15 s to 5 min. IAV infectivity was measured by plaque assay using MDCK cells. a The representative pictures of plaques of No UV, 2 min, and 5 min are shown. Dilution factors of IAV are displayed on the top of each well. b The titers are plotted on the graph and shown as pfu/mL (●). Assays were repeated four times and values represent the mean ± standard error. No plaque formation was observed after 4 and 5 min of irradiation and these were defined as not detected (ND). The ratio of experiments in which plaque formation was observed is also plotted on the graph (◆)