Table 2.

Comparison of sensitivities of the long-range RT-qPCR with the conventional RT-qPCR and the plaque assay

	RT-qPCR (copies/ml)	Normalized to plaque assay (%)
PB2	5.7 × 107	261
PA	5.4 × 106	24
HA	5.3 × 108	2395
NA	6.8 × 108	3109
NP	4.5 × 108	2041
M	3.6 × 108	1627
NS	1.5 × 108	700
Conventional RT-qPCR	9.6 × 109	43,636

Original copy numbers measured in the RT-qPCRs were divided by that in the conventional RT-qPCR or the titer in the plaque assay. Values are shown as percentages. PB2, PA, HA, NA, NP, M, and NS indicates the long-range RT-qPCR for PB2, PA, HA, NA, NP, M, and NS, respectively. Normalization was performed as follows: RT-qPCR (copies/ml) / Plaque assay (2.2 × 107 pfu/ml)