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. 2020 Jul 14;10(7):1045. doi: 10.3390/biom10071045

Figure 3.

Figure 3

DNA synthesis activity of PolB and PolD on linear substrates. (A) Kinetics of reaction for extension of the 5′end labeled 29 nt for the linear D-loop S91/29/30 or primer/template S29/91. (B) Strand displacement activity of DNA polymerases. A quantity of 225 nM of PolB or PolD was incubated with 25 nM synthetic linear DNA substrates at 65 °C for a range of time indicated. DNA products were separated by gel electrophoresis onto a 15% denaturing acrylamide gel and revealed by fluorescence.