Figure 2.

Dynamic changes of mononuclear cell subpopulations in wt muscles during regeneration. (A) The different mononuclear cell samples from uninjured and CTX-injured wt hind limb muscles were merged to create a single t-distributed stochastic neighbor embedding (t-SNE) map; colored according to expression levels of SCA1, CD90.2, α7-integrin, CD146, CD34, CD25, CD45, CD31, CD206, F4/80, CD140β, CXCR4, vimentin, and CD140α (blue: low expression; red: high expression of the selected marker). (B) FlowSOM heatmap of column normalized (Z-score) marker expression for each of the 15 identified clusters. Colors varied according to the expression level of the considered marker in a blue to red scale, indicating low to high expression, respectively. (C) Cell clusters defined by the FlowSOM analysis were assigned to arbitrary colors (yellow: immune cells; light blue: macrophages; pink: myogenic progenitors (MPs); dark green: fibro/adipogenic progenitors (FAPs); red: endothelial progenitors; blue: pericyte-like cells; light green: mesenchymal-like cells; purple: other). (D) Time course of the variation in cell subpopulation abundance upon CTX-induced injury. The dynamic changes were illustrated by density plots, colored according to cell density (blue: low density; red: high density).