Figure 5.

Characterization of the population dynamics induced by CTX injury in the mdx skeletal muscle at day 1, 3, 5, 10, 20 after injury. (A) Bar plots, showing the CD45^- (blue) / CD45⁺ (red) ratio in the mdx mononuclear cells at different time points after CTX injury. Data were represented as mean, and the statistical significance was estimated by two-way ANOVA. (B) t-SNE maps, showing the population gated as macrophage, colored for CD45 and F4/80 expression (red: high expression; blue: low expression) together with the macrophage trend observed in uninjured and CTX-injured mdx hind limb muscles. (C) M2 macrophages, expressing the CD206 antigen. (D) t-SNE maps of the myogenic progenitor (MP) population colored according to the levels of α7-integrin expression (red: high expression) and MP population dynamics during regeneration. (E) t-SNE maps, representing fibro/adipogenic progenitors (FAPs) identified among uninjured and CTX-injured mdx hind limb muscles, colored according to the expression of SCA1, CD34, CD140α, CD90.2, and vimentin (red: high expression; blue: low expression). The bar plot illustrated the population dynamics during regeneration. (F) Bar plots, showing the abundance of two different subclusters (cluster 1 and cluster 6) of endothelial progenitor cells at different time points. (G) t-SNE maps of pericyte-like cells identified in uninjured and CTX-injured mdx hind limb cell populations. Cell clusters were colored according to the expression of CD146, α7-integrin, and CD140β (red: high expression; blue: low expression). The bar plot illustrated the population dynamics during regeneration. Population abundance was assessed by calculating the percentage of cells in any given population over the total number of mononuclear cells in each sample (n = 3). The statistical significance was estimated by one-way ANOVA. All data were represented as mean ± SEM, and the statistical significance was defined as * p < 0.05; ** p < 0.01; **** p < 0.001.