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. 2020 Jul 7;13(7):144. doi: 10.3390/ph13070144

Figure 5.

Figure 5

Ectopic upregulation of miR-101-3p in low expressing breast cancer cells reduces BC trans-endothelial migration.MDA-MB-231-TGL and MDA-MB-231-BrM2 cells were transfected with miR-101-3p mimic (5 nM final concentration) or with scrambled control. (A) Relative miR-101-3p level expression was measured by real time PCR from total RNA. The small nuclear RNA U6 was used as internal control. (B) The transmigration abilities of the different BC cells were examined by the trans-endothelial migration assay. (C) Representative images of transmigrated fluorescently labelled MDA-MB-231-TGL and MDA-MB-231-BrM2 (small green cells) in control and miR-101-3p mimic transfected cells. * p < 0.05, ** p < 0.01, *** p < 0.001.