Table 1.
Summary of SH3-binding WIP epitopes.
| Partner | WIP Segment | Binding Motif | Effect | Ref. |
|---|---|---|---|---|
| Cortactin SH3 (NPF) | 136–205 | Not determined (ND) | Increases cortactin’s activation of the Arp2/3 complex, cortactin recruits WIP in invadopodium formation | [75,92] |
| mAbp1 SH3 (adaptor) |
110–170 | ND | Regulates dorsal ruffle formation | [93] |
| ITSN1-S/ ITSN1-L 1st/3rd/5th of 5 SH3 domains (adaptor) |
318–450 | ND | enhances association of ITSN1 with N-WASp and β-actin, facilitates formation of filopodia-like protrusions, regulates intra-cellular vesicle trafficking | [95,100] |
| ITSN2 1st/3rd/5th of 5 (adaptor) |
13–450 | ND | [95,96] | |
| CrkL 1st SH3 of 2 (adaptor) |
321–415 |
332PRLPQR337 (class 2) 399PQLPSR404 (class 2) (comply with Crk SH3.1 consensus binding motif PxLPxK/R) |
Presumably preformed CrkL-WIP-WASp complex associates with phos-ZAP70 after T cell receptor (TCR) ligation | [15] |
| Nck-1 2nd SH3 of 3 (adaptor) |
247–261 328–342 |
247SNRPPLPPTPSRALD261 328NDETPRLPQRNLSLS342 (both class 2, 328–342 complies with the consensus motif for Nck SH3.2 PxxPxRxxS) |
Couples extracellular signals to cytoskeleton assembly system | [26,98,99] |