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. 2020 Jul 15;9(7):1696. doi: 10.3390/cells9071696

Figure 2.

Figure 2

FACS analysis on fresh and cryopreserved hAEC: (A) a representative gating strategy is represented in panel A, where forward and side scatters identified intact cells, an additional gate selects single cells, later gated for different surface markers using two different fluorochromes. The negative isotype control for the respective fluorochromes is shown in the small dot plot graph. (B) Cells positive for CD49f or CD326 before (grey bars) and after cryopreservation (black bars). (C) Amnion-derived mesenchymal stromal cells (MSC) were identified based on their expression of characteristic markers (CD44 and CD105), before (grey bars) and after cryopreservation (black bars). (D) The hematopoietic marker CD45 was measured on cells in suspensions obtained after isolation (grey bars) and after cryopreservation (black bars). All values measured in 14 preparations are reported, with an average + SD as the last bar on the right. * p < 0.001, t-test on paired values.