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. 2020 Jul 15;12(7):3953–3963.

Figure 3.

Figure 3

CircMAGI3 targeted miR-515-5p/HDGF axis. A. Subcellular location analysis illustrate the location of circMAGI3 in the cytoplasm or nuclear in NSCLC cells. B. RT-PCR revealed the expression of multiple miRNAs after circMAGI3 was knocked down. C. The wild type and mutant sequences of circMAGI3 were constructed. D. Luciferase reporter assay found the close connection of circMAGI3 wild type with miR-515-5p. E. Online bioinformatics predictive tools revealed the wild type and mutant sequences of HDGF mRNA 3’-UTR. F. Luciferase reporter assay found the close connection of HDGF mRNA 3’-UTR wild type with miR-515-5p. G. RT-PCR showed the HDGF mRNA expression in A549 cells transfected with miR-515-5p mimics or inhibitor (inh). H. RT-PCR showed the HDGF mRNA expression in A549 cells transfected with circMAGI3 overexpression or miR-515-5p mimics. *P < 0.05, **P < 0.01, data represent the means ± SD.