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. 2020 Jul 15;12(7):3871–3883.

Figure 4.

Figure 4

HIF-1α silencing suppressed the development of HCC by negatively regulating NEAT1 level in Hep3B and SK-Hep1 cells under hypoxia. (A, B) Migration rates were detected in Normal (non-transfected and non-hypoxia control), Hypoxia, pc-Control (non-targeting and hypoxia control), pc-NEAT1 (NEAT1 transfected and hypoxia), pc-NEAT1 + siHIF-1α (NEAT1 and siRNA-HIF-1α co-transfected under hypoxia), siHIF-1α groups in Hep3 (A and B) and SK-Hep1 cells (C and D) by scratch wound test. (E-H) Invasion rates were detected in Normal, Hypoxia, pc-Control, pc-NEAT1, pc-NEAT1 + siHIF-1α, siHIF-1α groups in Hep3 (E and F) and SK-Hep1 cells (G and H) by transwell assay. ***P < 0.001 vs. Normal. ###P < 0.001 vs. pc-Control. ^^^P < 0.001 vs. pc-NEAT1. ΔΔΔP < 0.001 vs. pc-NEAT1 + siHIF-1α. The data were shown as mean ± standard deviation (S.D.). Abbreviations: HIF-1α, hypoxia-inducible factors-1α; NEAT1, lncRNA nuclear-enriched abundant transcript 1.