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. 2020 Jul 15;12(7):3871–3883.

Figure 5.

Figure 5

Knockdown of HIF-1α inhibited tumor growth in vivo through negatively regulating NEAT1 expression. A. Photographs of representative tumor diameter of nude mice. B. Immunohistochemistry assays were performed to detect HIF-1α protein expression in tumor tissues of Control (no injection of Hep3B cells), siHIF-1α (injection of Hep3B cells transfected with siRNA HIF-1α), siHIF-1α + pc-NEAT1 (injection of Hep3B cells transfected with siRNA HIF-1α and NEAT1) and pc-NEAT1 (injection of Hep3B cells transfected with NEAT1) groups. C. Tumor volumes were measured in tumor tissues of siHIF-1α, siHIF-1α + pc-NEAT1 and pc-NEAT1 groups. D. Tumor weights were measured in tumor tissues of siHIF-1α, siHIF-1α + pc-NEAT1 and pc-NEAT1 groups. E. The mRNA levels of NEAT1 were determined in tumor tissues of siHIF-1α, siHIF-1α + pc-NEAT1 and pc-NEAT1 groups by qRT-PCR. GAPDH served as an internal control. ***P < 0.001 vs. Control. ###P < 0.001 vs. siHIF-1α. ^^^P < 0.001 vs. siHIF-1α + pc-NEAT1. The data were shown as mean ± standard deviation (S.D.). Abbreviations: HIF-1α, hypoxia-inducible factors-1α; NEAT1, lncRNA nuclear-enriched abundant transcript 1; qRT-PCR, quantitative reverse transcription-polymerase chain reaction.