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Changes in TRAIL secretion with day and expression of HSV-TK in engineered MSCs. To evaluate the amount of secreted TRAIL, 20 MOI of rAd/dTRAIL-TK was transduced on MSCs with 50 µmol/L of Fe3+ for 30 minutes. Changes in amounts of secreted TRAIL were measured at days indicated, with GCV (A) or without GCV (B). After rAd/dTRAIL-TK was transduced on MSCs for 48 hours, the expression level of mRNA of HSV-TK was analyzed by reverse transcriptase PCR (C).
