Figure 2.

The combination of DOX and apatinib enhances apoptosis in MDA-MB-231/DOX cells. A. MDA-MB-231/ADR cells treated with 10 μM apatinib, 20 μM DOX or the combination of DOX and apatinib for 24 h were analyzed by Annexin V/PI staining. B. Cells were stained with PI and then analyzed by flow cytometry. C. Activation levels of caspases and PARP (cleavage bands) were determined with western blot. The data shown are representative of 3 independent experiments. Data are presented as means ± SD (n = 3). **P<0.01 vs the indicated group.