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. 2020 Jul 15;12(7):3501–3511.

Figure 5.

Figure 5

NLRC5 is a novel target of miR-125b-5p in breast cancer cells. (A) The predicted potential binding sites of miR-125b-5p in the 3’-UTR of NLRC5. (B) Luciferase activity was detected in MCF-7 and MDA-MB-231 cells transfected with pGL3-NLRC5-3’-UTR-WT and pGL3-NLRC5-3’-UTR-Mut reporter vectors, along with miR-125b-5p or miR-NC. (C) The western blot assay was used to characterize NLRC5 expression in MCF-7 and MDA-MB-231 cells with or without miR-125b-5p. The protein levels of NLRC5 were determined in breast cancer cells and MCF-10A cells (D) as well as in breast cancer tissues and paired normal tissues (E). *P < 0.05; **P < 0.01.