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The loss of XIST induced NLRC5 repression as XIST usually binds to miR-125b-5p in breast cancer cells. A. NLRC5 expression was quantified using qRT-PCR at 48 h after si-NC, si-XIST, si-XIST+anti-miR-NC, or si-XIST+anti-miR-125b-5p were transfected into MCF-7 and MDA-MB-231 cells. B. Protein expression levels of NLRC5 in the transfected cells were determined using the western blot assay. *P < 0.05; **P < 0.01.
