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. 2020 Jul 15;12(7):3429–3444.

Figure 7.

Figure 7

Overexpression of LINC01021 reverses Da0324-mediated cytotoxic effects in gastric cancer cells. A. BGC823 and SGC7901 cells were transfected with negative control vector (pLVX-NC) or overexpressing LINC01021 vector (pLVX-LINC01021) for 48 h. LINC01021 expression was determined by qRT-PCR analysis. The data represent the mean ± SD from three independent experiments. ***, P < 0.001. B. BGC823 and SGC7901 cells were transfected with pLVX-NC or pLVX-LINC01021 for 48 h. Cell viability was evaluated by CCK-8 assays. The data represent the mean ± SD from three independent experiments. **, P < 0.01; ***, P < 0.001. C, D. Overexpression of LINC01021 reversed Da0324-mediated growth inhibition of gastric cancer cells. BGC823 and SGC7901 cells were transfected with pLVX-NC or pLVX-LINC01021 for 24 h and then treated with Da0324 (4 µM) for 24 h. Cell viability was determined by CCK-8 assays. The data represent the mean ± SD from three independent experiments. *, P < 0.05; ***, P < 0.001. E. BGC823 and SGC7901 cells transfected with pLVX-NC or pLVX-LINC01021 were exposed to Da0324 for 48 h and colony formation was assessed. F. BGC823 and SGC7901 cells were transfected with pLVX-NC or pLVX-LINC01021 for 48 h and the expression levels of P53, Bcl-2, E-cadherin, N-cadherin and vimentin were determined by western blot analysis. GAPGH was used as an internal control. qRT-PCR, quantitative reverse transcription-polymerase chain reaction.