Figure 2.

Knockdown of circ_BICD2 suppressed the malignant biological behavior of both OSCC cells. A. RT-qPCR was used to examine the expression of circ_BICD2 in both SCC25 and Ca9-22 cells when transfected with circ_BICD2 shRNA or shRNA control. B. CCK-8 assay was applied to evaluate the cell proliferation. C and D. Colony formation assay was used to evaluate the colony formation rate. E-H. The migration and invasion capacity of OSCC cells using Transwell assay. I-K. Western blot was performed to measure the protein levels of E-cadherin, N-cadherin and Vimentin. Data are presented as means ± SD of three independent experiments. *P<0.05, **P<0.01, ***P<0.001, vs the sh-NC group.