Figure 3.

Inhibition of circ_BICD2 decreased the Warburg effect in both SCC25 and Ca9-22 cells. A. The glucose uptake colorimetric assay kit was used to detect the levels of glucose uptake in both SCC25 and Ca9-22 cells when transfected with circ_BICD2 shRNA or knockdown of circ_BICD2 plus 2-DG addition. B. The lactate colorimetric assay kit was applied to measure the level of lactate production. C. The ATP colorimetric assay kit was performed to examine the level of ATP generation in both SCC25 and Ca9-22 cells. Data are presented as means ± SD of three independent experiments. **P<0.01, ***P<0.001, vs the control group; ^#P<0.05, vs the 2-DG+sh-BICD2. sh-BICD2: circ_BICD2 shRNA; 2-DG: glycolytic inhibitor.