Figure 1.

Platelet hyperactivation is detected in CCA and CCA cell-platelet aggregates. A. Flow cytometric analysis of the concentration of PMPs in whole blood. The plasma was diluted with annexin V binding buffer and incubated with antibodies against annexin V (eBioscience) and CD41. Equal amounts of 1 µm beads (Invitrogen) were added to the sample as a size standard and analysed by a FACSVerse flow cytometer. B. Representative immunofluorescence staining of HCCC-9810 and RBE cell-platelet aggregates as described in the Materials and Methods. ****P<0.0001.