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. 2020 Jul 15;12(7):3577–3595.

Figure 5.

Figure 5

PDGF upregulates the expression of MMP2/MMP9 and induces EMT by activating the p38MAPK pathway. A. Transwell migration and invasion assays of control HCCC-9810 and platelet-stimulated HCCC-9810 cells with or without SB inhibitors (20 μmol/l) incubation for 12 h and 36 h. B. Transwell migration and invasion assays of control RBE and platelet-stimulated RBE cells with or without SB inhibitors (20 μmol/l) incubation for 12 h and 24 h. C. P-P38MAPK, P38MAPK, MMP2 and MMP9 protein expression levels were determined by western blotting in control HCCC-9810 and platelet-stimulated HCCC-9810 cells with or without SB inhibitors (20 μmol/l) incubation for 12 h. D. P-P38MAPK, P38MAPK, MMP2 and MMP9 protein expression levels were determined by western blotting in control RBE and platelet-stimulated RBE cells with or without SB inhibitors (20 μmol/l) incubation for 24 h. E. P-P38MAPK, P38MAPK, E-cadherin, N-cadherin, Slug, Snail, ZEB1 and vimentin protein expression levels were determined by western blotting in control HCCC-9810 and platelet-stimulated HCCC-9810 cells with or without SB inhibitors (20 μmol/l) incubation for 12 h. F. P-P38MAPK, P38MAPK, E-cadherin, N-cadherin, Slug, Snail, ZEB1 and vimentin protein expression levels were determined by western blotting in control RBE and platelet-stimulated RBE cells with or without SB inhibitors (20 μmol/l) incubation for 24 h. *P<0.05; **P<0.01; ***P<0.001 and ****P<0.0001.