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. 2020 Jul 15;12(7):3660–3673.

Figure 3.

Figure 3

miR-424 directly targets 3’-UTR of Apelin and APJ genes. A. The 3’-UTR of the Apelin gene used for construction of recombinant plasmids for dual-luciferase reporter assays. B. Direct targeting of the 3’ UTR of Apelin gene by miR-424 in human KGN cells. C. The binding sites of miR-424 on the Apelin gene was predicted by TargetScanHuman 7.1. D. The 3’-UTR of the APJ gene used for construction of recombinant plasmids for dual-luciferase reporter assays. E. Direct targeting of 3’-UTR of APJ by miR-424 in human KGN cells, shown by dual-luciferase reporter assays. F. Binding of miR-424 to 3’ UTR of APJ gene predicted by TargetScanHuman 7.1 software. APJ: Apelin receptor; NC: negative control; WT: wild type; MUT: mutant; UTR: untranslated region; * and ** indicates P < 0.05 and < 0.01, respectively.