Figure 5.
miR-424 promotes KGN cell apoptosis by inducing Caspase-3 truncation. A. Alteration of KGN cell apoptosis induced by transfection with miR-424 mimics and inhibitors. Cell apoptosis was analyzed by flow cytometry. B. Quantitation of apoptotic KGN cells transfected with miR-424 mimics and inhibitors. C. The abundance of Caspase-3 protein and truncated Caspase-3 protein in KGN cells transfected with miR-424 mimics and inhibitors. Western blotting was performed to analyze protein abundance. NC: negative control; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; *P < 0.05.