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. 2020 Jul 10;10(7):1030. doi: 10.3390/biom10071030

Figure 2.

Figure 2

Effects of AMP/SUL treatments (100 mg/kg and 200 mg/kg, i.p.) for five days on the expression of GLT-1 in the AcbCo and AcbSh. (A) Immunoblot of GLT-1 and β-tubulin in the AcbCo. (B) Quantitative analysis using one-way ANOVA followed by Newman-Keuls test indicated that there were no significant differences in the expression of GLT-1 among water-control, ethanol-control, ethanol-AMP/SUL (100 mg/kg), and ethanol-AMP/SUL (200 mg/kg) in the AcbCo. (C) Immunoblot of GLT-1 and β-tubulin in the AcbSh. (D) Quantitative analysis using one-way ANOVA followed by Newman-Keuls test indicated that there was a significant decrease in GLT-1 expression in the ethanol control group compared to water-control group, while post-treatments with AMP/SUL (100 and 200 mg/kg) upregulated GLT-1 expression compared to ethanol-control group in the AcbSh. Ethanol-AMP/SUL (100 and 200 mg/kg) groups had lower GLT-1 expression compared to water-control group. The symbol of statistical significance between any group and water-control group is shown on the bar of the group. Water-control group data were represented as 100% (relative to water-control). The values are expressed as mean ± SEM (n = 5/group), (* p < 0.05, ** p < 0.01 and $ p < 0.001).