Figure 2.

Binding of selected clones to ED-LDLR in various experimental conditions. (A) Apparent dissociation constants (K*_d) of the 13 representative clones selected from the phage display experiments. Clones with low K*_d, revealing high affinities and selected for further characterization, are in green. (B–D) K*_d of the clones 40, 41, and 47 in normal conditions, at acidic pH (pH 6.0, pH 5.0), and in the absence of calcium (Ca²⁺ free). (E–G) Ratios between K*_d in modified conditions and K*_d of the clones, reflecting the inhibitory effects of these modifications on the clones binding. (H) Percentage of dissociation of the clones from the LDLR.