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. 2020 Jul 16;12(7):667. doi: 10.3390/pharmaceutics12070667

Figure 2.

Figure 2

IL-6 knockdown in dividing ARPE-19 cells, 3 days after transfection. Black columns indicate the IL-6 siRNA (50 nM), the white columns indicate the negative control siRNA (50 nM). IL-6 protein secretion was evaluated with ELISA and was normalized to LPS-treated cells (100%, column with diagonal pattern). The basal IL-6 secretion of dividing ARPE-19 cells was 5% relative to the LPS-treated cells. L2000: Lipofectamine 2000; ctrl: negative control siRNA; * indicates statistical significance (p < 0.05). Data are presented as mean + SD, n = 3–4.