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Intracellular distribution of ATTO565-labelled dsDNA (in red) in the 2-week matured hESC-RPE. dsDNA (50 nM) was incorporated into the commercial carriers DF4 (a) and Metafectene PRO (b), and into lipidoids (c). Lysosomes were labelled with CellLight Lysosomes-GFP (in green). Cell nuclei (in blue) were labelled with DAPI. A: apical side; B: basolateral side. Scale bar: 10 μm.
