Table 1.
Gene Name | Primer Pairs | Melting Tm (°C) |
---|---|---|
β-actin | Forward 5′-CAATAGTGATGACCTGGCCGT-3′ Reverse 5′-AGAGGGAAATCGTGCGTGAC-3′ |
60 |
NLRP1B | Forward 5′-AGGCAAGCACATTTGACTTCAAGGT-3′ Reverse 5′-GCTCAGTAGCTGCATCTTGCATTTC-3′ |
60 |
NLRC4 | Forward 5′-GCGGAGGTGGGAGATATG-3′ Reverse 5′-CGTAGAAGGTTTGGAACAGC-3′ |
60 |
Caspase-1 | Forward 5′-GGACTCTCAGCAGCTCCTCAGGCA-3′ Reverse 5′-GCAAAGCTTGACATTCCCTTCTGAGCC-3′ |
60 |
IL-1β | Forward 5′-GACCTTCCAGGATGAGGACA-3′ Reverse 52032-AGGCCACAGGTATTTTGTCG-3′ |
60 |
IL-18 | Forward 5′-ACAGTGAAGTAAGAGGACTGGCTG-3′ Reverse 5′-CAGGTGCATCCATTTCCTCAAAGG-3′ |
60 |
GSDMD | Forward 5′-CCAGTGCCTCCATGATGAATGTGT-3′ Reverse 5′-TCACCACAAACAGGTCATCCC-3′ |
60 |