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. 2020 Jul 5;10(7):1001. doi: 10.3390/biom10071001

Table 1.

Primer pairs of candidate genes used in real time quantitative PCR.

Gene Name Primer Pairs Melting Tm (°C)
β-actin Forward 5′-CAATAGTGATGACCTGGCCGT-3′
Reverse 5′-AGAGGGAAATCGTGCGTGAC-3′
60
NLRP1B Forward 5′-AGGCAAGCACATTTGACTTCAAGGT-3′
Reverse 5′-GCTCAGTAGCTGCATCTTGCATTTC-3′
60
NLRC4 Forward 5′-GCGGAGGTGGGAGATATG-3′
Reverse 5′-CGTAGAAGGTTTGGAACAGC-3′
60
Caspase-1 Forward 5′-GGACTCTCAGCAGCTCCTCAGGCA-3′
Reverse 5′-GCAAAGCTTGACATTCCCTTCTGAGCC-3′
60
IL-1β Forward 5′-GACCTTCCAGGATGAGGACA-3′
Reverse 52032-AGGCCACAGGTATTTTGTCG-3′
60
IL-18 Forward 5′-ACAGTGAAGTAAGAGGACTGGCTG-3′
Reverse 5′-CAGGTGCATCCATTTCCTCAAAGG-3′
60
GSDMD Forward 5′-CCAGTGCCTCCATGATGAATGTGT-3′
Reverse 5′-TCACCACAAACAGGTCATCCC-3′
60