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. 2020 Jul 28;32(4):107959. doi: 10.1016/j.celrep.2020.107959

Figure 2.

Figure 2

Caspase-1 and Caspase-8 promote Activation of Caspase-3 and Caspase-7 during Pyroptosis

(A and B) Macrophages of the indicated genotypes were left untreated or received TAT-Cre (as described in STAR Methods), and subsequently received FlaTox (A) or LeTx (B) or were left untreated in media containing the caspase-3/7 activity (DEVD) probe, and were imaged on an Incucyte platform.

(C–F) Macrophages of the indicated genotypes were left untreated or stimulated with LeTx (C), FlaTox (D, E), or log-phase S. Typhimurium (F) in media containing the caspase-3/7 activity (DEVD) probe and imaged on an Incucyte platform.

Results from Incucyte experiments are plotted as the number of positive cells relative to a PI-stained, Triton-x100-treated well (considered 100%). Values represent mean ± SD of technical duplicates of a representative experiment from three biological repeats.