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. 2020 Jul 28;32(4):107959. doi: 10.1016/j.celrep.2020.107959

Figure 4.

Figure 4

Caspase-1 and Caspase-8 Act Redundantly in GSDMD-Deficient Macrophages for Activation of Caspase-3 and Caspase-7

(A–C) Macrophages of the indicated genotypes were left untreated or stimulated with either LeTx or FlaTox for 2 h. Cells were imaged under a confocal microscope (A) or analyzed by FACS for Annexin-V/PI positivity (B), and cell lysates were immunoblotted for the indicated proteins (C).

(D and E) Macrophages of the indicated genotypes were left untreated or stimulated with either LeTx (D) or FlaTox (E) in media containing DEVD probe and PI and imaged on an Incucyte platform.

(F and G) Macrophages of the indicated genotypes were left untreated or stimulated with either LeTx (F) or FlaTox (G) in media containing DEVD probe and PI and imaged on an Incucyte platform.

(H) Macrophages of the indicated genotypes were left untreated or stimulated with either LeTx or FlaTox for 2 h, and cell lysates were immunoblotted for the indicated proteins.

Percentages of all Incucyte experiments were calculated as the number of positive cells relative to a PI-stained, Triton-x100-treated well (considered 100%). Values represent mean ± SD of technical duplicates of a representative experiment from three biological repeats. All scale bars represent 10 μm.