REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal anti-caspase-1	Adipogen	Cat# AG-20B-0042-C100; RRID: AB_2755041; clone Casper-1
Mouse monoclonal anti-caspase-8	Enzo Life Sciences	Cat# ALX-804-447-C100; RRID: AB_2050952; clone 1G12
Rabbit monoclonal anti-cleaved caspase-8 (Asp387)	Cell Signaling	Cat# 8592S; RRID: AB_10891784; clone D5B2
Rabbit polyclonal anti-caspase-3	Cell Signaling	Cat# 9662; RRID: AB_331439
Rabbit monoclonal anti-cleaved caspase-3 (Asp175)	Cell Signaling	Cat# 9664S; RRID: AB_2070042; clone 5A1E
Rabbit polyclonal anti-caspase-7	Cell Signaling	Cat# 9492; RRID: AB_2228313
Rabbit polyclonal anti-cleaved caspase-7 (Asp198)	Cell Signaling	Cat# 9491; RRID: AB_2068144
Rabbit polyclonal anti-ASC	Adipogen	Cat# AG-25B-0006; RRID: AB_2490440
Rabbit monoclonal anti-GAPDH	Cell Signaling	Cat# 14C10; RRID: AB_561053; clone 2118
Rabbit monoclonal anti-ROCKI	Abcam	Cat# ab45171; RRID: AB_2182005; clone EP786Y
Goat polyclonal anti-Bid	R&D systems	Cat# AF860; RRID: AB_2065622
Goat polyclonal anti-mouse, HRP-conjugated	Jackson Immunoresearch Laboratories	Cat# 115-035-146; RRID: AB_2307392
Goat polyclonal anti–rabbit, HRP-conjugated	Jackson Immunoresearch Laboratories	Cat# 111-035-144; RRID: AB_2307391
Mouse monoclonal anti-β-actin, HRP-conjugated	US Biological	Cat# 137402.100
Bacterial and Virus Strains
Salmonella enterica serovar Thypimurium, SL1344	In house	N/A
Chemicals, Peptides, and Recombinant Proteins
LFn-FlaA	In house	(von Moltke et al., 2012)
B. anthracis protective antigen (PA)	In house	(von Moltke et al., 2012)
B. anthracis lethal factor (LF)	List Biologicals	Cat# 172C
TAT-Cre	In house	(Peitz et al., 2002)
LPS-SM	Invivogen	Cat# tlrl-smlps
MG132	Calbiochem	Cat# 474791
polymyxin B sulfate	Calbiochem	Cat# 5291
staurosporine	Selleckchem	Cat# S1421
cycloheximide	Sigma-Aldrich	Cat# C4859
leupeptin	Sigma-Aldrich	Cat# L8511
chloroquine	Sigma-Aldrich	Cat# C6628
β-estradiol	Sigma-Aldrich	Cat# E2758
ATP	Sigma-Aldrich	Cat# ATPD-RO
Enhanced chemiluminescence solution	Thermo Scientific	Cat# 32106
propidium iodide	Thermo Scientific	Cat# P1304MP
Sytox Green	Thermo Scientific	Cat# S7020
DEVD-based substrate	Thermo Scientific	Cat# R37111
Critical Commercial Assays
CytoTox 96 non-radioactive cytotoxicity assay	Promega	Cat# G1780
annexin V-FITC and PI	BD PharMingen	Cat# 556547
Experimental Models: Cell Lines
ER-Hoxb8 immortalized myeloid progenitor cells	In house	(Wang et al., 2006)
Experimental Models: Organisms/Strains
Mouse: B6: C57BL/6J	In house	Charles River
Mouse: B6Nlrp1b+: C57BL/6JNlrp1b+Tg/WT	In house	(Boyden and Dietrich, 2006)
Mouse: Casp1−/−/Casp11−/−	In house	(Kuida et al., 1995)
Mouse: Nlrc4−/−		(Mariathasan et al., 2004)
Mouse: Bcl2Tg: C57BL/6JH2K-Bcl2Tg/WT	In house	(Domen et al., 1998)
Mouse: Gsdmd−/−	In house	(Kayagaki et al., 2015)
Mouse: GSDMDI105N: C57BL/6J - GSDMD I105N/I105N	In house	(Kayagaki et al., 2015)
Mouse: Ripk3−/−Casp8−/−	In house	(Newton et al., 2014)
Mouse: Asc−/−	In house	(Mariathasan et al., 2004)
Mouse: Casp3/7flox/flox: C57BL/6J - Casp3flox/flox/Casp7flox/flox	In house	(Saavedra et al., 2018)
Mouse: Casp3/7Myel-KO: C57BL/6J - Casp3flox/floxCasp7flox/floxLysM-Cre+	In house	(Clausen et al., 1999; Saavedra et al., 2018)
Software and Algorithms
Fiji	NIH	N/A
FlowJo	FlowJo LLC	N/A
Prism 8	GraphPad	N/A
Incucyte Zoom	Essenbio	N/A