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. 2020 Jul 7;12(7):641. doi: 10.3390/pharmaceutics12070641

Figure 3.

Figure 3

Cytotoxic effect of N-AI PAI-2 liposomes on breast cancer spheroids. (a) Spheroid diameter was measured after incubation with EMP PAI-2 liposomes or N-AI PAI-2 liposomes with MCF-7 and MDA-MB-231 multicellular tumor spheroids, over a period of 96 h. (b) Representative bright-field images and (c) fluorescent images, following the addition of calcein-AM to visualize the viable cells were captured at the same magnification (n = 3). Spheroids in (b, c) were treated with 25 µM N-AI or the equivalent concentration of phospholipid in liposomal formulation. Scale bars are 100 µm. Data are the mean ± s.d. (n = 3).