Figure 1.

NK cells promoted DC uptake of TNBC cells opsonized with Cetuximab. Cytotoxicity of IIB-BR-G cells (left) and their uptake by DCs (right) after a co-culture of DCs with IIB-BR-G cells opsonized with 10 µg/mL isotype control (IC) or Cetuximab in the absence (DCs) or presence of NK cells (DCs+NK) at a DCs:NK:IIB-BR-G ratio of 1:1:1 for 4 h at 37 °C. (A) Upper panel: simplified gating strategy for CFSE⁺ IIB-BR-G cells (left) and CD11c⁺ DCs (right). Lower panel: for the different experimental conditions, representative dot plots showing tumor cell lysis (% of 7AAD⁺ cells within CFSE⁺ tumor cells) are presented in the left, and tumor cell uptake by DCs (% of CFSE⁺ cells within the CD11c⁺ DCs) in the right. (B) Bars with different letters are statistically different, p < 0.05 (ANOVA) (n = 3).