Figure 2.

Robust cell viability in the 3D adipose construct. (A–D) Constructs were processed for Live (calcein, green)/Dead (EthD-1, red) staining after 28 days in culture. (A,B) Static cultures; (C,D) dynamic cultures (scale bar, 1 mm); (A,C) growth medium; and (B,D) adipogenic medium. There was increased EthD-1 positive (dead) staining in the dynamic constructs as compared to the static constructs, while no apparent differences were observed between the cultures in the growth medium versus the adipogenic medium. (E) The area positive for Calcein staining over a total area demonstrated a significant decrease in the cell viability in dynamic cultures as compared to static cultures (N = 3, ** p < 0.01, *** p < 0.001). (F) Cell proliferation assessed by the Alamar blue staining of static constructs after 7, 14, 21, and 28 days of culture in growth media (N = 3). Cell proliferation was seen over the four-week culture period. EthD-1, ethidium homodimer-1.