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. 2020 Jul 17;10(7):1070. doi: 10.3390/biom10071070

Figure 4.

Figure 4

Increased production of ADIPOQ and LPL in the cultures maintained in the adipogenic medium. ADIPOQ and LPL were assayed by ELISA after 28 days in culture. (A) ADIPOQ concentration was lower in the static and dynamic 3D bioreactor culture when compared with the monolayer culture (N = 4, **** p < 0.0001). (B) The LPL concentration increased in the cultures maintained in the adipogenic medium and comparably between the monolayer and static 3D culture (N = 4, * p < 0.05). (C) LPL level increased in the 3D cultures upon maintenance in the adipogenic medium (N = 4, * p < 0.05). It should be noted that the culture medium from the 3D dynamic cultures required concentrating to achieve the measurable LPL levels. GM, growth medium; AQ; adipogenic medium.