Figure 1.

Denguenil inhibits dengue virus (DENV) transcript levels. (A) Schematic representation of zebrafish model of dengue viral pathology. Human serum infected with dengue virus was propagated in primary carrier fish. Study zebrafish (n = 24) were injected with dengue virus (DENV-3) (Disease Control) or PBS as in control (Normal Control) and treated with three different effective doses (5.6 μg/kg, 28 μg/kg, and 140 μg/kg Denguenil) for various time points. (B) Whole RNA from liver tissue was analyzed by quantitative real-time PCR (qPCR) for the dengue viral load. Virus induced increases in the DENV-3 copy number were decreased upon Denguenil treatment. n = 24, data are presented as means ± SEM. **, ##, #^ p < 0.001, * Represents significant compared to Normal Control; ##, # Represents significant compared to their respective Disease Controls; #^ Represents significant among two time points.