Delivery of antigens by targeting antibodies directed against select endocytic receptors expressed on DC subpopulations induces defined T cell responses in mice in vivo. Coupling of antigens to monoclonal targeting antibodies specifically recognizing endocytic dendritic cell (DC) surface receptors enable the loading of select DC subpopulations with an antigen of choice following in vivo application. While targeting antigens to murine cross-presenting cDC1 via DEC205, CLEC9A, Langerin (CD207), and CD36 were described to result in major CD8+ T cell responses following peptide:MHC-I presentation, antigen delivery via DCIR2 and CLEC10A expressed on cDC2 induce a strong CD4+ T cell response. Moreover, simultaneous delivery of antigens to cDC1 and cDC2 via FcγRIIB, FcγRIII, FcγRIV, CD11c, and MHC-II allow for the induction of concomitant CD8+ and CD4+ T cell responses. DC = Dendritic cell; cDC = conventional dendritic cell; MHC = major histocompatibility complex; DCIR = Dendritic cell immunoreceptor; CLEC = C-type lectin receptor; CD = cluster of differentiation; FcγR = Fc gamma receptor. The cellular images are provided and adapted from Servier Medical Art (smart.servier.com). The images are licensed under a Creative Commons Attribution 3.0 Unported License (creativecommons.org/licenses/by/3.0/).