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. 2020 Jul 2;9(7):1602. doi: 10.3390/cells9071602

Figure 3.

Figure 3

Effects of the miR-640 inhibitor on cell survival, migration, and differentiation. (A and B): HUVECs were transfected with control (LNA-scrambled) or miR-640 (LNA-anti-miR-640) inhibitors. Equal numbers of cells were cultured in complete, and basal medium containing PBS or Ang-1. Cell counts and cleaved caspase-3 optical densities (OD) were evaluated 24 h later. Values are means ± SEM. * p < 0.05, compared to cells maintained in complete medium. # p < 0.05, compared to PBS. (C): Scratch wound healing assays of HUVECs transfected with control or miR-640 inhibitors. Cells were maintained in basal medium containing PBS or Ang-1. Percentage wound healing was measured 8 h after wounding with a pipette tip. Values are means ± SEM. * p < 0.05, compared to PBS. # p < 0.05, compared to cells transfected with control inhibitor and treated with PBS or Ang-1. (D): Total tube numbers of HUVECs transfected with control or miR-64 inhibitors and maintained for 24 h in plates pre-coated with growth factor-reduced Matrigel and basal medium containing PBS or Ang-1. Values are means ± SEM. * p < 0.05, compared to PBS. # p < 0.05, compared to cells transfected with control inhibitor and treated with PBS or Ang-1.